Expanding the genetic code for site-specific labelling of tobacco mosaic virus coat protein and building biotin-functionalized virus-like particles----Technical Institute of Physics and Chemistry

A method for site-specific and high yield modification of tobacco mosaic virus coat protein (TMVCP) utilizing a genetic code expanding technology and copper free cycloaddition reaction has been established, and biotin-functionalized virus-like particles were built by the self-assembly of the protein monomers.

Chemical Communications . 03/2014; DOI:10.1039/c3cc49137d

Fig. 1 Genetic incorporation of KPN into TMVCP

Fig. 2 TEM images of the self-assembly structures of KPN11-TMVCP

Fig. 3 Site-specific modification and assembly of KPN11-TMVCP